REAL-TIME PCR TECHNOLOGY FOR CATTLE GENOTYPING BY A AND B KAPPA-CASEIN GENE ALLELES

Аннотация

The main goal of the study was to develop and test an effective technology for cattle genotyping by
the CSN3 gene based on real-time PCR with hybridization-fluorescence detection. There was developed a method for
real-time PCR for cattle genotyping by A and B alleles of the CSN3 gene in the format of hybridization-fluorescence
detection, involving the use of two 5/
-fluorescence-labeled forward allele-specific primers, one reverse common
primer, and one anti-primer labeled with a fluorescence quencher at the 3/
-end of the oligonucleotide. As a result of
practical studies aimed at testing the developed method, we obtained the technical result provided by the proposed
technology, expressed in the effective identification of the desired genotypes due to correct interpretation of these
curves of increasing fluorescence intensity, the results reliability of which was also confirmed by the well-known
PCR-RFLP analysis technique for Bos taurus genotyping for similar allelic variants of the kappa-casein gene