MOLECULAR GENETIC PLANT ANALYSIS, ARTEMISIA L. GENUS, WITH ISSR-MARKERS

Аннотация

Molecular genetic analysis of three plants of thistle family has been carried out in the scope of this
paper (Artemisia karatavica Krasch. & Abolin ex Poljakov, Artemisia cina Berg ex Poljakov, Artemisia porrecta
Krasch. ex Poljakov). The plants have been collected in Turkestan region, Shardarinsky district, 15 km north-east of
the village Komsomol, and along the road in Turkestan region, Baydibeksky district, 4.5 km south-east of the village
Shakpak, and in the steppes in Turkestan region, Aryssky district, 1 km north-east of the village Darmino. In this
paper, we used modern methods of molecular biology in order to determine genetic relatedness.
ISSR analysis using universal primers has been conducted. ISSR-markers are the most common markers, and
they are used for phylogenic analysis. This method is based on amplification of sequences limited by two
microsatellite repeats using the primer that is complementary to the sequence of this microsatellite (4-12 repeat
units). ISSR (region of the genome between two adjacent, oppositely oriented microsatellites) the sequence of
microsatellite medullar part with some (1-3) nucleotides adjacent to the repeat tandem are used as primers. Tens of
fragments of locus variety received in PCR are separated by electrophoresis and assessed for the presence or absence
(due to marker dominance) of the fragments of a particular size. The main advantage of this type of markers - lack of
necessity for knowledge of the sequences during primer designing.