@article{R. R. Vafin_Kh. Kh. Gilmanov_A. G. Galstyan_N. S. Pryanichnikova_A. V. Bigaeva_E. G. Lazareva_V. S. Kazakova_2021, title={TECHNOLOGY OF BOVINE LEUKEMIA VIRUS GENODIAGNOSTICS IN CATTLE, IN PRODUCED RAW MATERIALS AND PRODUCTS}, url={https://journals.nauka-nanrk.kz/chemistry-technology/article/view/351}, abstractNote={<p>The most important task of the dairy cattle industry is to obtain high quality raw milk. To achieve it,<br>a set of measures is required, including aimed at increasing the biological safety of produced raw materials. The aim<br>of the study was to create a scientific and methodological basis for the Bovine leukemia virus (BLV) gene diagnostics<br>in a combined format of pathogen indication and identification. This required updating the strategy of BLV<br>PCR-RFLP genotyping, consistent with its phylogenetic classification, taking into account the growing knowledge<br>about the genetic diversity of 11 genotypes of the studied viral pathogen. When staging nested PCR, oligonucleotide<br>primers were used, which initiate at the final stage of the reaction the production of a 444 bp env-gene fragment of<br>the pathogen. Five restriction endonucleases were used in PCR-RFLP BLV genotyping of: PvuII, SspI, AsuHPI,<br>HaeIII, and BstX2I. As a result of verification of the developed Bovine leukemia virus method for gene identification<br>with an updated genotyping strategy, a technical result was obtained, expressed in the ability to identify all 11 BLV<br>genotypes discovered to date by interpreting the generated 58 genotype-associated combinations of PCR-RFLP<br>profiles</p>}, number={1}, journal={Известия НАН РК. Серия химии и технологии}, author={R. R. Vafin and Kh. Kh. Gilmanov and A. G. Galstyan and N. S. Pryanichnikova and A. V. Bigaeva and E. G. Lazareva and V. S. Kazakova}, year={2021}, month={фев.}, pages={119–125} }